TEGEX - cell types and gene expression in the developing tegmentum





Here you can visualize gene expression in single cell RNA sequencing data of the mouse embryonic tegmentum/anterior brainstem using Seurat tools (https://satijalab.org/seurat/).

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The data have been generated in Partanen lab Molecular and Integrative Biosciences Research Programme

Faculty of Biological and Environmental Sciences

University of Helsinki





Created for Partanen lab by Sami Kilpinen and Lassi Virtanen

BACKGROUND


The tegmentum/anterior brainstem contains multiple types of glutamatergic and GABAergic neurons associated with the monoaminergic systems and important for regulation of mood, motivation and movement.


These neurons are derived from the ventral rhombomere 1.


Our research aims at comprehensive characterization of

  1. Anterior brainstem GABAergic and glutamatergic cell types
  2. Gene expression characterizing them
  3. Cellular and molecular mechanisms regulating their development


References:

Achim et al., Mechanisms regulating GABAergic neuron development. Cell Mol Life Sci 2014, PMID: 24196748

Lahti et al., Differentiation and Molecular heterogeneity of inhibitory and excitatory neurons associated with midbrain dopaminergic nuclei. Development 2016, PMID: 26718003

EXPERIMENTS


Single-cell RNA sequencing (Chromium 10xGenomics) of the cell types in the embryonic (E12.5 and E13.5) ventral rhombomere 1




The main cell clusters identified in the E12.5 ventral r1


Plot 1




Plot 2

PUBLICATION


Francesca Morello# , Daniel Borshagovski# , Mantas Survila# , Laura Tikker# ,

Samir Sadik-Ogli, Anna Kirjavainen, Nuri Estartús, Laura Knaapi, Laura Lahti,

Petri Törönen, Linas Mazutis, Alessio Delogu, Marjo Salminen, Kaia Achim, Juha Partanen.


# Equal contribution

Molecular fingerprint and developmental regulation of the tegmental GABAergic and glutamatergic neurons derived from the anterior hindbrain

CONTACT


Juha Partanen

juha.m.partanen@helsinki.fi


Kaia Achim

kaia.achim@helsinki.fi


Molecular and Integrative Biosciences Research Programme

Faculty of Biological and Environmental Sciences

University of Helsinki